What can single molecule localisation microcopy (SMLM) do for you?
Walter and Eliza Hall Institute
1g Royal Parade, Parkville
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One would think that nothing is better than imaging single molecule to identify molecular mechanisms in intact and live cells. Indeed, single molecule localisation microscopy (also known as PALM, (d)STORM, GSDIM, PAINT etc.) promises to revolutionise molecular and cell biology as it provides a truly molecular perspective of complex cellular progresses. However, these SMLM approaches do not actually return images but the molecular coordinates of fluorescent events. The challenge now is to figure out how we can utilise these unique data sets to capture for example the functional heterogeneities in receptor signalling. Professor Gaus will provide an overview of the state-of-the-art of SMLM and SMLM data analysis, and illustrate how to use SMLM to better understand T cell signalling.
Registration is not required.