Professor Separovic has degrees in Maths & Physics from Macquarie University and UNSW. She was awarded the 2009 ASB Robertson Medal and 2011 ANZMAG Medal, and is a Fellow of the Biophysical Society, and the Australian Academy of Science. In 2017 she was named an IUPAC Distinguished Woman of Chemistry/Chemical Engineering and was inducted in the Victorian Honour Roll of Women in 2018. Her research involves determining the structure and dynamics of membrane components using solid-state NMR spectroscopy. She used them to help solve the molecular structure of the antibiotic gramicidin A and the bee toxin melittin within phospholipid membranes.